|
KMID : 0894520200240030207
|
|
Development & Reproduction
2020 Volume.24 No. 3 p.207 ~ p.214
|
|
|
Establishment of Conditions for Long-Term Maintenance of Primary Embryonic Cell Cultures from Olive Flounder Paralichthys olivaceus
|
|
Kim Ju-Won
Cho Ja-Young
Kim Dong-Gyun
Nam Bo-Hye
Nho Eun-Soo
Kim Bong-Seok
Kim Young-Ok
Kong Hee-Jeong
|
|
|
Abstract
|
|
|
|
Primary cell culture is a sufficient method frequently used to study the cellular properties and mechanisms of isolated cells in a controlled environment. In this study, an embryonic cell line (FGBC8) derived from the blastula stages of embryos of olive flounder Paralichthys olivaceus was developed. Furthermore, conditions for optimal long-term maintenance of this primary embryonic cell culture were investigated. Morphologically, FGBC8 cells were composed primarily of epithelial-like cells. FGBC8 cells were subcultured for >160 passages over ~830 days. The doubling time of FGBC8 cells was 73.8 h, and the modal diploid chromosome number was 48. FGBC8 cells transfected with green fluorescence protein (GFP)-expression plasmid exhibited a strong signal 48 h after transfection. Consequently, we demonstrated that fish serum is a crucial supplement for the long-term survival and maintenance of comparable morphology in these primary embryonic cells. Our results can be used as a guide for primary embryonic cell cultures for other fish species and may be useful for cell biotechnological applications.
|
|
|
KEYWORD
|
|
|
Olive flounder, Embryo, Primary cell culture, Long-term maintenance
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|